Matrix Metalloproteinase-9 Gene Polymorphism in Patients with Hepatocellular Carcinoma

Document Type : Original Article

Authors

1 Department of Biochemistry, Faculty of Science, Port-Said University.

2 Department of Clinical Pathology, Faculty of Medicine, Mansoura University.

3 Internal Medicine Department, Oncology Center, Faculty of Medicine, Mansoura University

4 Department of Medical Biochemistry, Faculty of Medicine, Port-Said University, Egypt.

5 Department of Biochemistry, Faculty of Science, Port-Said University

Abstract

Background: The focus of the study was to investigate the gene polymorphism of MMP-9 in hepatocellular cancer patients. Hepatocellular carcinoma is one of the most prevalent cancers. Matrix metalloproteinases are enzymes that degrade extracellular matrix proteins and are essential for cell migration during cancer invasion. The frequency of MMP-9 1562 C/T (3918242) was determined in HCC patients and the level of MMP-9 protein and its variation in HCC progression were evaluated in this study. Method: The study was conducted in a cohort of 100 samples (50 patients with HCC with cirrhosis and 50 patients with hepatitis C virus infection as controls). MMP-9 1562 C/T was determined by PCR-RFLP and MMP-9 protein level were assessed by ELISA. Result: In HCC patients P = 0.001, indicated a significant difference in the TT genotype between the patient group when compared to HCV group and the T allele had a high statistical significance among cancer patients (p = 0.001). Comparing MMP-9 protein levels between the control group and cancer patients identified a significant variation in MMP-9 protein level (p = 0.02). Conclusions: According to the data, the development of hepatocellular carcinoma was positively correlated with MMP-9. Hepatocellular carcinoma prognosis and prediction may be greatly affected by MMP-9.

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Alfarama Journal of Basic & Applied Sciences
Volume 4, Issue 3
July 2023
Pages 413-422

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