Document Type : Original Article
Authors
1
Chemistry department,faculty of science,port said university,port said ,egypt
2
Chemistry Department, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt
3
Plant Protection Research Institute, Agriculture Research Center, 12618, Egypt
4
Chemistry Department, Faculty of Science, Port Said University, Port Said, Egypt
Abstract
Alhagi graecorum methanolic crude extract and its derived fractions (methylene chloride (mc), n-hexane (nh), n-butanol , and ethyl acetate finally the residual aqueous fraction) ;all were examined for their potential antioxidant activity performing the Superoxide dismutase like activity besides DPPH (α-diphenyl-β-picrylhydrazyl) assays. Moreover, the antibacterial property towards different pathogenic microbial Gram-positive ;( Staphylococcus aureus,(BS) Bacillus subtilis) in addition to Gram-negative strains(Proteus volgaris(PV), and Escherichia coli(EC)). Identification of the secondary volatile metabolites of both Alhagi graecorum hexane and methylene chloride fractions was by Gas Chromatography/Mass Spectra (GC-MS) method. The identified extracted volatile constituents could be mainly categorized to two main classes, oxygenated acetogenins (fat derivatives) and oxygenated terpenes. Twelve volatile constituents were characterized in the hexane fraction, the most dominant compounds were methyl oleate (36.07%), methyl hexadecanoate (17.32%), methyl 11E,14E-octadecadienoate (13.32%). Of the eighteen constituents identified in the methylene chloride fraction, the most dominant were 4-(3-hydroxy-2,2,6-trimethyl-7-oxa-bicyclo[4.1.0]hept-1-yl)-but-3-en-2-one (5.51%), 2-dodecen-1-ylsuccinic anhydride (7.24%) and (–)-loliolide (19.73%).
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