Survey of marine-derived fungal biota and efficacy of its metabolites as plant growth promoter

Tawfik Ali ElHaty, Moataz Mohamed; Kardousha, Alaa Hassan; Ibrahim, Mohsen; Mansour, Mohamed

doi:10.21608/ajbas.2022.147309.1114

Survey of marine-derived fungal biota and efficacy of its metabolites as plant growth promoter

Document Type : Original Article

Authors

¹ Department of Botany, Faculty of Science, Port Said University, Port Said, 42524, Egypt.

² Botany Department, Faculty of science, port Said University, Egypt

³ Botany Department, Faculty of Science, Port Said University

⁴ department of botany and microbiology, Faculty of science, Al-Arish University, Egypt

10.21608/ajbas.2022.147309.1114

Abstract

Marine fungi inhabiting algae represent an ecologically and taxonomically interesting group of microorganisms. Because the algal host is the best known for fungi, the information on fungi associated with algae is necessary for discovering secondary metabolites. Marine macroalgae associated with the fungal communities like Ulva Lactuca linnaeus, Enteromorpha intestinalis Linnaeus, and Dictyota sp were carried out for surveying fungal biota. Those algae were collected from Suez Canal and seashore in the Mediterranean Sea at Port Said governorate. The study aims to investigate the role of these fungi and their secondary metabolites on plant growth. The most abundant fungal communities are phylum Ascomycota and genera Penicillium, Cladosporium, Fusarium, and Aspergillus.26, marine fungi were isolated from algal samples and culturing on plates with Czapek’s yeast extract agar amended with chloramphenicol and Rose Bengal. After identification of organisms by macroscopic and microscopic observation, isolated fungi are let to produce natural secondary metabolites into the media after a week, 50% ethyl acetate in water was added to broth media, then incubated at room temperature. The crude extract was collected after using a separating funnel for solvent separation and using rotavapor for extraction. Secondary metabolites were identified using GC-MS analysis as Linalool from Trichoderma harzainum while Methyl jasmonate and Nylidrin from Penicillium crustosum. Raw secondary metabolites of the taxa Penicillium crustosum and Trichoderma harzainum give increasing in length of shoot and root system of (eggplant) Solanum melongena and also affects the whole plant by increasing its dry weight.

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