Solid-state hydrolysis and fermentation of rice straw and manure for bioethanol production

Document Type : Original Article

Authors

1 Botany Department, Science Faculty, PortSaid University, Ismailia, Egypy.

2 Department of Botany, Faculty of Science, Port Said University, Port Said, 42524, Egypt.

3 Department of Botany, Faculty of Science, University of Suez Canal, Ismailia, Egypt

Abstract

Bioethanol production from lignocellulose biomass is a suitable alternative to replace fossil fuels. In Egypt, rice straw and manure are the large resources of lignocellulosic biomass from agriculture wastes and animal wastes, respectively. Solid-state hydrolysis and fermentation utilizing rice straw and manure, using co-culture of Streptomyces aegyptia and Candida tropicalis, were investigated. The response surface methodology (RSM) was used to determine the optimal medium volume in hydrolysis and fermentation processes for maximum ethanol production. The maximum ethanol concentration of 4.4 g/l (22 mg/g) was produced with medium volume in hydrolysis and fermentation processes of 1.5 and 3.5 ml/g, respectively. The efficiency of bioconversion processes not only depends on conditions used for cultivation but also depends upon the efficiency of strains used to utilize the lignocelluloses. UV mutagenesis of Streptomyces aegyptia was considered in this study. Results showed different effects among the lignocellulose enzymes measured in this study. Strain exposed to UV light (280-320 nm) at a distance of 10 cm for 20 seconds was the best one for sugar (57.3 mg/g) and bioethanol production 5.8 g/l (29 mg/g).

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