A Comparative Study: Gene Expression VEGF, FLT-1and KDR And level of PLGF In Preeclampsia And Normal Pregnancy.

M. EL-Deen, Ibrahim; Seleem, Amal; Alrefaey, Abdel Aziz; Elsayed, Elsherbiny; Amin, Ateya Gomaa

doi:10.21608/ajbas.2020.37520.1028

A Comparative Study: Gene Expression VEGF, FLT-1and KDR And level of PLGF In Preeclampsia And Normal Pregnancy.

Document Type : Original Article

Authors

¹ Department of Chemistry, Faculty of Science, Port Said University, Port Said, Egypt.

² Department of Clinical Biochemistry, Faculty of Medicine

³ Department of Gynocology, Faculty of Medicine, Mansoura University, Mansoura, Egypt. .

⁴ Insurance of health

10.21608/ajbas.2020.37520.1028

Abstract

involved The vascular endothelial growth factor (VEGF) family of angiogenic growth factors in the pathophysiology of these inconveniences . aims This study to examine Maternal serum levels of
placental growth factor (PLGF) and Placental mRNA expression of VEGF, fms-like tyrosine kinase 1( FLT-1) and kinase insert domain receptor (KDR) in normotensive control (NC) women (n = 24) and women with preeclampsia (PE) (n = 50) [25 severe PE (SPE) and 25 Mild PE (MPE)]. We conducted a cross-sectional study in 50 preeclamptic women: 25women with mild PE, 25 patients with severe PE. While 24 normotensive pregnant women matched for age as a control group. we measured by real time PCR (RTPCR) Placental mRNA expression of VEGF FLT-1and KDR . and we measured Maternal serum levels of PLGF by ELISA. there was statistically significant difference between the three study groups as regards all 4 studied laboratory variables. KDR, FLT-1 and PLGF were all statistically significantly higher in control group as compared to either of the two PE groups. The mean VFGF expression was statistically higher in cases of severe PE than Mild PE, both were higher than control group (p < 0.0005).

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